This manual provides operating instructions for the cay adams1 2000 series centrifuges the single speed 2001 and the two speed symbol definitions the following is an explanation of the syrnbo s found throughout the manual and located on the centrifuge unts. View and download bd accuri c6 instrument manual online. Accuri c6 laboratory equipment pdf manual download. Breakthrough with the bd accuri c6 plus cell analyzer. Cited in over 9,000 publications, the bd accuri c6 plus enables you to go beyond your current research potential. In addition, we use bd accuri c6 flow cetometer 15012010 make, but bought it on 2014. Finally, the plant dna analysis template was opened. Bd accuri c6 software controls the bd accuri c6 flow cytometer system in order. The bd accuri c6 flow cytometer system includes bd accuri c6 software for controlling the instrument, generating statistics, and analyzing results. Aurora cytek aurora is a spectral analyzer with five lasers 355 nm, 405 nm, 488 nm, 561 nm, 640 nm, three light scattering channels, and 64 fluorescence channels. Consult bd s entire sero fuge catalogue on medicalexpo. It is possible to measure some other fluorochromes using this setup. The bd accuri c6 flow cytometer figure 2 offers performance, simplicity, and affordability.
Bd accuri c6 software user guide manual vi 782009501 rev0 table b1. Performance validation of the bd accuri c6 was then done using 6 and 8peak fluorescent bead mixtures spherotech according to instructions bd accuri c6 software user guide. The bd accuri c6 plus personal flow cytometer is the newest generation of the bd accuri platform. Bd accuri c6 plus software files can be exported in fcs 3. By default, bd accuri c6 software displays the area parameter signified with a suffix of a, but parameters can also be displayed in height, width of primary threshold channel, only, or time. Bd accuri c6 software user guide manual 66 782009501 rev0 select instrument extended clean of flow cell. To streamline sample processing, the bd csampler allows multiple. Clickfree c6 user manual bd accuri c6 software user guide manual. Biolegends legendplex is a beadbased immunoassay that uses the principles of sandwich elisas to quantify soluble analytes using a flow cytometer. In addition, c6 has a flow metering system for automatic calculation of absolute cell counts.
Instructional video on the basics of using byus accuri c6 cytometer. The problems started around may of 2017, almost 3 years after usage. If you have questions or require further clarification, see the bd accuri c6 flow cytometer instrument manual or contact accuri technical support. It also includes common troubleshooting help and service information. Click here to view the bd fluorescence spectraviewer. Many bd accuri c6 customers can begin collecting and analyzing data with the help of a quick start guide. A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file.
Direct counts on the bd accuri c6 plus correlate highly with counting beads and are more precise than manual counts. Accuri cytometers c6 flow cytometer community, manuals. The bd accuri c6 is a digital basic flow cytometer, which is equipped with a blue 488nm and a red 640nm lasers, two light scatter detectors fsc and ssc, and four fluorescence detectors. For the d accuri 6, use the pe channel fl2 for the reporter and the. Accuri cytometers c6 flow cytometer community, manuals and. Bd accuri c6 software user guide imunologia e imunopatologia 14.
Plate setup for ending a data collection session 80. Bd accuri c6 plus flow cytometer optical filter guide. Turn on the computer and log in with your ucf info. Bd facscanto ii medical equipment user manual manual pdf view. Only operate the accuri c6 flow cytometer with an original ed version of software authorized by accuri. Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field.
Accuri c6 maintenance cleaning and decontamination. The accuri csampler is an optional accessory for the c6 flow cytometer. Install, update, and run antivirus protection software on a regular basis. Instrument manual bd accuri c6 instrument manual 31 pages. The intuitive interface of the software guides the user through workflows. Enhanced sensitivity, reliability, and capabilities bring flow.
Donald breakwellfilmed and edited by gabriel goulding. The bd accuri c6 flow cytometer analyser can be used after training. We have 1 bd accuri c6 manual available for free pdf download. Bd accuri c6 flow cytometer instrument manual bd accuri c6 software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed. Bd pyxis es refrigerator imx105 operation user manual 360393 rev b this manual is for end users of the imx105 bd pyxis es refrigerator. Cytometry bd accuri question hi all, as always, the list has been. Highsensitivity, lownoise avalanche photodiode detectors, a sensor for measuring in real time the sample flow rate. See bd accuri c6 software users guide, chapter 1, the basics for a complete description of how to run and analyze bead results. Setup procedure for d accuri 6 this part of the guide applies to bd digital flow cytometers using d accuri 6 software.
Follow the installation instructions in the wizard. Fortessa cell analyser full user guide bd facsdiva software quick reference guide for fortessa. Bd bd accuri c6 plus community, manuals and specifications. Core research instrumentation school of pharmacy and. Any one have good or bad experiences with the new bd accuri.
Bd accuri c6 plus software features an intuitive user interface. Accuri c6 is an easy to use flow cytometer which can be connected to an autosampler 48 and 96 well, and 24 tube rack, we do not have one. Bd csampler software user guide iv 782009901 rev2 table of figures figure 11. The bd accrui c6 cytometer the cytometer has 2 lasersblue 488nm laser 3 colorsred 640 1 colorunlike most cytometers you will encounter, the accuri does not all you to manually adjust your voltage settingsinstead, the software uses a larger logarithmic scale and auto. When setting up the cytometer, note the details in the following sections to maximize the instruments performance. Bd accuri c6 flow cytometer instrument manual 7820018 rev2 3 refer to the bd accuri c6 safety and limitations manual pn 7820091 for complete safety information. Msds linked at the right cleaning solution, kr225 citric acid. The selectable laser module allows 3 different filter configurations. For the d accuri 6, use the pe channel fl2 for the reporter and the apc channel fl4 for beads classification. Blue 488nm laser 3 colors red 640 1 color unlike most cytometers you will encounter, the accuri does not all you to manually adjust your voltage settings instead, the software uses a larger logarithmic scale and autofits events onto the plots for you.
Valve issues, fluidics leaking onto electronics, flow cell. Bd facsaria ii users guide hfgcc bd facscalibur users guide wolf hall bd accuri c6 users guide wolf hall bd online introduction to flow cytometry web training. Even new flow cytometry users find it so easy to learn and use that. Assemble the computer according to the manufacturers instructions. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on youtube.
The unique fluidics system of the bd accuri c6 plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. Bd facsdiva software quick reference guide for hts option hts sampler daily operation tutorial bd cytometer setup and tracking application guide. Bd accuri c6 with single tube loader protocol this protocol contains instructions for setting up an accuri c6 with tube loader. Using the bd accuri c6 cytometer for rapid and accurate. Bd accuri c6 flow cytometer quick set up guide please follow these directions for setting up your bd accuri c6 flow cytometer. Bd facscanto ii medical equipment user manual manual pdf. Bd accuri c6 software user guide manual ii 782009501 rev0 4. The bd accuri c6 plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the singlecell level. Bd accuri c6 flow cytometers, bd cflow software and workstation pc supply all the capabilities of a fullfeatured flow cytometer at an affordable price. Accuri c6 is a digital pc based bench top analyser, 2 laser system.
Bd accuri c6 with plate handler april 2016 this protocol contains instructions for setting up an accuri c6 with plate handler. Calibrationshould be performedin the same tube type as the experimental sample. To fill the cleaning solution bottle green line, add 3 ml of 1030% citric acid and fill with di. The accuri csampler is an optional accessory for the c6 flow cytometer that. If you are interested in using a fluorochrome that is not listed here with the accuri c6, please contact the operator click here to view the instrument manual. Bd accuri c6 instrument manual pdf download manualslib. This includes removal and replacement of the peristaltic pump tubing, inline sheath filter and the fluidic bottle filters. When calling the technical support of bd, i was told that old generation can only run on 32bit. Carefully inspect all cartons upon receipt of the cytometer. Bd accuri c6 plus flow cytometer with its compact 11 x 14. Within 5 minutes of completing the water run, place a calibration sample on the sip. Operate the instrument as described in this instruction manual.
Bd accuri c6 flow cytometer instruction manual, serial number 2400 and higher. When combined with the portfolio of bd reagents and bestinclass service and support, it is an ideal solution for life science researchers performing routine assays in cellular analysis, while also. This portable and compact system holds the analytical power of a cytometer twice its size. Biolegends legendplex kits are provided with predefined panels, ranging from 3 to specificities, or customers can mix and match any subset within each predefined panel using our mix and match system. The bd intraosseous manual driver is the only intraosseous manual drill that features an integrated passive needle tip safety designed to decrease needlestick injuries. Bd accuri c6 software will display this notification based on whichever trigger is reached first. Simultaneously you can analyse with 4 different fluorescent and 2 scatter parameters. Compare with other higher end bd instrument autosamplers, this one does not seem very effective at keeping cells is suspension or picking them up without regular manual. Take a leap in flow cytometry with the bd accuri c6 plus. Even more colors to choose from with selectable lasers and filter replacement. The software for accuri c6 cflow plus is very user friendly and efficient. The system includes blue and red lasers, four color detectors, and both forward and side scatter detectors plus software that is so intuitive that you will typically be up and running within.
C6 flow cytometer instrument manual bd accuri c6 software user guide manual. Please contact us to schedule time on the instrument, set up a training appointment or to discuss a future project. Bd facsdiva software quick reference guide for hts option. Standard fluorochrome in use are identical to facs calibur. A guide to absolute counting using the bd accuri c6 flow cytometer bd biosciences august 2011 page 3 5. The bd accrui c6 cytometer university of washington. All users are responsible for obtaining the proper approval and training before the use of the bd accuri c6 flow cytometer. Jul 22, 2016 accuri c6 consumables consumables fluids. If you are interested in using a fluorochrome that is not listed here with the accuri c6, please contact the operator. Bd accuri c6 flow cytometer instrument manual 7820018 rev4 1 1 introduction the bd accuri c6 flow cytometer offers an entirely new vision for the role of flow cy tometry in life science research. The bd accuri c6 plus offers simplified workflows and readytouse templates for performing a wide range of applications in flow cytometry.
Fixed optics and capillary sheathflow fluidics make the bd accuri c6. Buy from accuri, or make your own citric acid solution. The bd accrui c6 cytometer the cytometer has 2 lasers. Click here to view the bd fluorescence spectraviewer please note that while the spectraviewer is a. Hi as far as i know, there is nothing that bd can do to overcome this issue on accuri c6 itself by bd claims that they have used larger valves and thicker tubing system in accuri c6 plus and facsvia ivd version to cope with this problem. Any one have good or bad experiences with the new bd. If you are the first user of the day, you can run for 15 minutes. This manual provides information on cytometer requirements and specifications, setup, and maintenance. Accuri c6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to facscalibur. The bd csampler is an optional accessory for the bd accuri c6 flow cytometer that allows for the collection of samples prepared in 48 and 96well plates and. The passive needle safety tip protects healthcare providers from inadvertent needlesticks in emergent situations. The instrument has a blue 488 nm and a red 640 nm laser.
User guides and information flow cytometry core facility. Bd accuri c6 cytometer manual getting ready ensure that the fluid levels in the sheath, cleaning, and decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Designed from the ground up to be compact and easy to use, the bd accuri c6 flow cytometer fits in any lab and is readily accessible. Default cytometer configuration for the accuri c6 analyzer.
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